Multifunctional magnetic bead-based electrochemical immunoassay for the detection of aflatoxin B1 in food.
Identifieur interne : 001D58 ( Main/Exploration ); précédent : 001D57; suivant : 001D59Multifunctional magnetic bead-based electrochemical immunoassay for the detection of aflatoxin B1 in food.
Auteurs : RBID : pubmed:20448920English descriptors
- KwdEn :
- Aflatoxin B1 (analysis), Aflatoxin B1 (immunology), Aflatoxin B1 (isolation & purification), Animals, Antibodies (immunology), Biosensing Techniques (methods), Cattle, Electrochemistry (methods), Electrodes (utilization), Enzyme-Linked Immunosorbent Assay (methods), Ferrocyanides (chemistry), Gold (chemistry), Horseradish Peroxidase (chemistry), Immunoassay (methods), Immunomagnetic Separation, Limit of Detection, Magnetics (methods), Nanoparticles (chemistry), Nanostructures, Nanotechnology, Tin Compounds (chemistry).
- MESH :
- chemical , analysis : Aflatoxin B1.
- chemical , chemistry : Ferrocyanides, Gold, Horseradish Peroxidase, Tin Compounds.
- chemical , immunology : Aflatoxin B1, Antibodies.
- chemical , isolation & purification : Aflatoxin B1.
- chemistry : Nanoparticles.
- methods : Biosensing Techniques, Electrochemistry, Enzyme-Linked Immunosorbent Assay, Immunoassay, Magnetics.
- utilization : Electrodes.
- Animals, Cattle, Immunomagnetic Separation, Limit of Detection, Nanostructures, Nanotechnology.
Abstract
A sensitive and reusable electrochemical immunoassay for aflatoxin B(1) (AFB(1)) in food has been developed. A multifunctional magnetic bead (MMB) was initially synthesized using magnetic CoFe(2)O(4) nanoparticle as the core and Prussian blue nanoparticle (PBNP)-doped silica as the shell, and then the prepared MMB was used as an affinity support for the immobilization of the AFB(1)-bovine serum albumin conjugate (AFB(1)-BSA). With the aid of an external magnet, the AFB(1)-BSA-conjugated MMBs were attached on the surface of an indium tin oxide (ITO) electrode. Gold nanoparticles, labeled with horseradish peroxidase (HRP)-bound anti-AFB(1) antibodies (HRP-anti-AFB(1)), were employed as detection antibodies. With a competitive immunoassay format, the concentrations of AFB(1) in samples were measured in PBS (pH 7.0) by using PBNP-doped MMBs as the mediator, HRP-anti-AFB(1) as the tracer and hydrogen peroxide (H(2)O(2)) as the enzyme substrate, and the linear range was 0.05-12 ng/mL with a detection limit of 6.0 pg/mL AFB(1) (at 3sigma). Intra- and inter-assay coefficients of variation were less than 7.5%. In addition, the content of AFB(1) in red paprika specimens has been assayed by the developed immunoassay and a commercially available enzyme-linked immunosorbent assay (ELISA) method, respectively, and consistent results were obtained. The as-prepared immunoassay provides a promising approach for the screening of organic pollutants because it is simple, rapid, highly sensitive, specific, and without the need of sample pre-concentration.
DOI: 10.1039/b902401h
PubMed: 20448920
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Multifunctional magnetic bead-based electrochemical immunoassay for the detection of aflatoxin B1 in food.</title><author><name sortKey="Tang, Dianping" uniqKey="Tang D">Dianping Tang</name><affiliation wicri:level="3"><nlm:affiliation>Chair for Analytical Chemistry, Institute of Hydrochemistry, Technische Universität München, Marchioninistrasse 17, D-81377 München, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Chair for Analytical Chemistry, Institute of Hydrochemistry, Technische Universität München, Marchioninistrasse 17, D-81377 München</wicri:regionArea><placeName><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Haute-Bavière</region><settlement type="city">Munich</settlement></placeName></affiliation></author><author><name sortKey="Zhong, Zhaoyang" uniqKey="Zhong Z">Zhaoyang Zhong</name></author><author><name sortKey="Niessner, Reinhard" uniqKey="Niessner R">Reinhard Niessner</name></author><author><name sortKey="Knopp, Dietmar" uniqKey="Knopp D">Dietmar Knopp</name></author></titleStmt><publicationStmt><date when="2009">2009</date><idno type="doi">10.1039/b902401h</idno><idno type="RBID">pubmed:20448920</idno><idno type="pmid">20448920</idno><idno type="wicri:Area/Main/Corpus">001940</idno><idno type="wicri:Area/Main/Curation">001940</idno><idno type="wicri:Area/Main/Exploration">001D58</idno></publicationStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aflatoxin B1 (analysis)</term><term>Aflatoxin B1 (immunology)</term><term>Aflatoxin B1 (isolation & purification)</term><term>Animals</term><term>Antibodies (immunology)</term><term>Biosensing Techniques (methods)</term><term>Cattle</term><term>Electrochemistry (methods)</term><term>Electrodes (utilization)</term><term>Enzyme-Linked Immunosorbent Assay (methods)</term><term>Ferrocyanides (chemistry)</term><term>Gold (chemistry)</term><term>Horseradish Peroxidase (chemistry)</term><term>Immunoassay (methods)</term><term>Immunomagnetic Separation</term><term>Limit of Detection</term><term>Magnetics (methods)</term><term>Nanoparticles (chemistry)</term><term>Nanostructures</term><term>Nanotechnology</term><term>Tin Compounds (chemistry)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Aflatoxin B1</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Ferrocyanides</term><term>Gold</term><term>Horseradish Peroxidase</term><term>Tin Compounds</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Aflatoxin B1</term><term>Antibodies</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Aflatoxin B1</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Nanoparticles</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biosensing Techniques</term><term>Electrochemistry</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Immunoassay</term><term>Magnetics</term></keywords><keywords scheme="MESH" qualifier="utilization" xml:lang="en"><term>Electrodes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cattle</term><term>Immunomagnetic Separation</term><term>Limit of Detection</term><term>Nanostructures</term><term>Nanotechnology</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A sensitive and reusable electrochemical immunoassay for aflatoxin B(1) (AFB(1)) in food has been developed. A multifunctional magnetic bead (MMB) was initially synthesized using magnetic CoFe(2)O(4) nanoparticle as the core and Prussian blue nanoparticle (PBNP)-doped silica as the shell, and then the prepared MMB was used as an affinity support for the immobilization of the AFB(1)-bovine serum albumin conjugate (AFB(1)-BSA). With the aid of an external magnet, the AFB(1)-BSA-conjugated MMBs were attached on the surface of an indium tin oxide (ITO) electrode. Gold nanoparticles, labeled with horseradish peroxidase (HRP)-bound anti-AFB(1) antibodies (HRP-anti-AFB(1)), were employed as detection antibodies. With a competitive immunoassay format, the concentrations of AFB(1) in samples were measured in PBS (pH 7.0) by using PBNP-doped MMBs as the mediator, HRP-anti-AFB(1) as the tracer and hydrogen peroxide (H(2)O(2)) as the enzyme substrate, and the linear range was 0.05-12 ng/mL with a detection limit of 6.0 pg/mL AFB(1) (at 3sigma). Intra- and inter-assay coefficients of variation were less than 7.5%. In addition, the content of AFB(1) in red paprika specimens has been assayed by the developed immunoassay and a commercially available enzyme-linked immunosorbent assay (ELISA) method, respectively, and consistent results were obtained. The as-prepared immunoassay provides a promising approach for the screening of organic pollutants because it is simple, rapid, highly sensitive, specific, and without the need of sample pre-concentration.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">20448920</PMID><DateCreated><Year>2010</Year><Month>05</Month><Day>07</Day></DateCreated><DateCompleted><Year>2010</Year><Month>08</Month><Day>10</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1364-5528</ISSN><JournalIssue CitedMedium="Internet"><Volume>134</Volume><Issue>8</Issue><PubDate><Year>2009</Year><Month>Aug</Month></PubDate></JournalIssue><Title>The Analyst</Title><ISOAbbreviation>Analyst</ISOAbbreviation></Journal><ArticleTitle>Multifunctional magnetic bead-based electrochemical immunoassay for the detection of aflatoxin B1 in food.</ArticleTitle><Pagination><MedlinePgn>1554-60</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1039/b902401h</ELocationID><Abstract><AbstractText>A sensitive and reusable electrochemical immunoassay for aflatoxin B(1) (AFB(1)) in food has been developed. A multifunctional magnetic bead (MMB) was initially synthesized using magnetic CoFe(2)O(4) nanoparticle as the core and Prussian blue nanoparticle (PBNP)-doped silica as the shell, and then the prepared MMB was used as an affinity support for the immobilization of the AFB(1)-bovine serum albumin conjugate (AFB(1)-BSA). With the aid of an external magnet, the AFB(1)-BSA-conjugated MMBs were attached on the surface of an indium tin oxide (ITO) electrode. Gold nanoparticles, labeled with horseradish peroxidase (HRP)-bound anti-AFB(1) antibodies (HRP-anti-AFB(1)), were employed as detection antibodies. With a competitive immunoassay format, the concentrations of AFB(1) in samples were measured in PBS (pH 7.0) by using PBNP-doped MMBs as the mediator, HRP-anti-AFB(1) as the tracer and hydrogen peroxide (H(2)O(2)) as the enzyme substrate, and the linear range was 0.05-12 ng/mL with a detection limit of 6.0 pg/mL AFB(1) (at 3sigma). Intra- and inter-assay coefficients of variation were less than 7.5%. In addition, the content of AFB(1) in red paprika specimens has been assayed by the developed immunoassay and a commercially available enzyme-linked immunosorbent assay (ELISA) method, respectively, and consistent results were obtained. The as-prepared immunoassay provides a promising approach for the screening of organic pollutants because it is simple, rapid, highly sensitive, specific, and without the need of sample pre-concentration.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Tang</LastName><ForeName>Dianping</ForeName><Initials>D</Initials><Affiliation>Chair for Analytical Chemistry, Institute of Hydrochemistry, Technische Universität München, Marchioninistrasse 17, D-81377 München, Germany.</Affiliation></Author><Author ValidYN="Y"><LastName>Zhong</LastName><ForeName>Zhaoyang</ForeName><Initials>Z</Initials></Author><Author ValidYN="Y"><LastName>Niessner</LastName><ForeName>Reinhard</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Knopp</LastName><ForeName>Dietmar</ForeName><Initials>D</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Journal Article</PublicationType><PublicationType>Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>05</Month><Day>20</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Analyst</MedlineTA><NlmUniqueID>0372652</NlmUniqueID><ISSNLinking>0003-2654</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Antibodies</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Ferrocyanides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Tin Compounds</NameOfSubstance></Chemical><Chemical><RegistryNumber>71243-84-0</RegistryNumber><NameOfSubstance>indium tin oxide</NameOfSubstance></Chemical><Chemical><RegistryNumber>7440-57-5</RegistryNumber><NameOfSubstance>Gold</NameOfSubstance></Chemical><Chemical><RegistryNumber>9N2N2Y55MH</RegistryNumber><NameOfSubstance>Aflatoxin B1</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.-</RegistryNumber><NameOfSubstance>Horseradish Peroxidase</NameOfSubstance></Chemical><Chemical><RegistryNumber>TLE294X33A</RegistryNumber><NameOfSubstance>ferric ferrocyanide</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N">Aflatoxin B1</DescriptorName><QualifierName MajorTopicYN="Y">analysis</QualifierName><QualifierName MajorTopicYN="N">immunology</QualifierName><QualifierName MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Antibodies</DescriptorName><QualifierName MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Biosensing Techniques</DescriptorName><QualifierName MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Cattle</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Electrochemistry</DescriptorName><QualifierName MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Electrodes</DescriptorName><QualifierName MajorTopicYN="N">utilization</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName><QualifierName MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Ferrocyanides</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Gold</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Horseradish Peroxidase</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Immunoassay</DescriptorName><QualifierName MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Immunomagnetic Separation</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Limit of Detection</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Magnetics</DescriptorName><QualifierName MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Nanoparticles</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Nanostructures</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Nanotechnology</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Tin Compounds</DescriptorName><QualifierName MajorTopicYN="Y">chemistry</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="aheadofprint"><Year>2009</Year><Month>5</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="epublish"><Year>2009</Year><Month>7</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>5</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>5</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>8</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="doi">10.1039/b902401h</ArticleId><ArticleId IdType="pubmed">20448920</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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